5W3R

SH2B1 SH2 Domain

5W3R の概要

• エントリーDOI: 10.2210/pdb5w3r/pdb
• 分子名称: SH2B adapter protein 1, PHOSPHATE ION, PHENOL, ... (4 entities in total)
• 機能のキーワード: sh2b1, sh2, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13041.74

構造登録者

McKercher, M.A.,Wuttke, D.S. (登録日: 2017-06-08, 公開日: 2017-11-22, 最終更新日: 2023-10-04)

主引用文献

McKercher, M.A.,Guan, X.,Tan, Z.,Wuttke, D.S.
Diversity in peptide recognition by the SH2 domain of SH2B1.
Proteins, 86:164-176, 2018

PubMed Abstract: SH2B1 is a multidomain protein that serves as a key adaptor to regulate numerous cellular events, such as insulin, leptin, and growth hormone signaling pathways. Many of these protein-protein interactions are mediated by the SH2 domain of SH2B1, which recognizes ligands containing a phosphorylated tyrosine (pY), including peptides derived from janus kinase 2, insulin receptor, and insulin receptor substrate-1 and -2. Specificity for the SH2 domain of SH2B1 is conferred in these ligands either by a hydrophobic or an acidic side chain at the +3 position C-terminal to the pY. This specificity for chemically disparate species suggests that SH2B1 relies on distinct thermodynamic or structural mechanisms to bind to peptides. Using binding and structural strategies, we have identified unique thermodynamic signatures for each peptide binding mode, and several SH2B1 residues, including K575 and R578, that play distinct roles in peptide binding. The high-resolution structure of the SH2 domain of SH2B1 further reveals conformationally plastic protein loops that may contribute to the ability of the protein to recognize dissimilar ligands. Together, numerous hydrophobic and electrostatic interactions, in addition to backbone conformational flexibility, permit the recognition of diverse peptides by SH2B1. An understanding of this expanded peptide recognition will allow for the identification of novel physiologically relevant SH2B1/peptide interactions, which can contribute to the design of obesity and diabetes pharmaceuticals to target the ligand-binding interface of SH2B1 with high specificity.

PubMed: 29127727
DOI: 10.1002/prot.25420

実験手法

X-RAY DIFFRACTION (1.386 Å)