5VZL

cryo-EM structure of the Cas9-sgRNA-AcrIIA4 anti-CRISPR complex

Summary for 5VZL

Entry DOI	10.2210/pdb5vzl/pdb
EMDB information	8749
Descriptor	single guide RNA (116-MER), CRISPR-associated endonuclease Cas9, phage anti-CRISPR AcrIIA4 (3 entities in total)
Functional Keywords	anti-crispr, cas9, crispr, gene editing, on-target, off-target, cryo-em, immune system-rna complex, immune system/rna
Biological source	Streptococcus pyogenes M1 GAS More
Total number of polymer chains	3
Total formula weight	207247.61
Authors	Jiang, F.,Liu, J.J.,Nogales, E.,Doudna, J.A. (deposition date: 2017-05-29, release date: 2017-07-26, Last modification date: 2024-03-13)
Primary citation	Shin, J.,Jiang, F.,Liu, J.J.,Bray, N.L.,Rauch, B.J.,Baik, S.H.,Nogales, E.,Bondy-Denomy, J.,Corn, J.E.,Doudna, J.A. Disabling Cas9 by an anti-CRISPR DNA mimic. Sci Adv, 3:e1701620-e1701620, 2017 Cited by PubMed: 28706995 DOI: 10.1126/sciadv.1701620 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.9 Å)

Structure validation