5VST

Crystal structure of murine CEACAM1b

「5F1D」から置き換えられました

5VST の概要

• エントリーDOI: 10.2210/pdb5vst/pdb
• 分子名称: Biliary glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: coronavirus, membrane protein, cell adhesion
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23666.39

構造登録者

Peng, G.,Yang, Y.,Pasquarella, J.R.,Xu, L.,Qian, Z.,Holmes, K.V.,Li, F. (登録日: 2017-05-12, 公開日: 2017-05-24, 最終更新日: 2024-10-30)

主引用文献

Peng, G.,Yang, Y.,Pasquarella, J.R.,Xu, L.,Qian, Z.,Holmes, K.V.,Li, F.
Molecular mechanism for coronavirus-driven evolution of mouse receptor
J. Biol. Chem., 292:2174-2181, 2017

PubMed Abstract: Hosts and pathogens are locked in an evolutionary arms race. To infect mice, mouse hepatitis coronavirus (MHV) has evolved to recognize mouse CEACAM1a (mCEACAM1a) as its receptor. To elude MHV infections, mice may have evolved a variant allele from the gene, called , producing mCEACAM1b, which is a much poorer MHV receptor than mCEACAM1a. Previous studies showed that sequence differences between mCEACAM1a and mCEACAM1b in a critical MHV-binding CC' loop partially account for the low receptor activity of mCEACAM1b, but detailed structural and molecular mechanisms for the differential MHV receptor activities of mCEACAM1a and mCEACAM1b remained elusive. Here we have determined the crystal structure of mCEACAM1b and identified the structural differences and additional residue differences between mCEACAM1a and mCEACAM1b that affect MHV binding and entry. These differences include conformational alterations of the CC' loop as well as residue variations in other MHV-binding regions, including β-strands C' and C'' and loop C'C''. Using pseudovirus entry and protein-protein binding assays, we show that substituting the structural and residue features from mCEACAM1b into mCEACAM1a reduced the viral receptor activity of mCEACAM1a, whereas substituting the reverse changes from mCEACAM1a into mCEACAM1b increased the viral receptor activity of mCEACAM1b. These results elucidate the detailed molecular mechanism for how mice may have kept pace in the evolutionary arms race with MHV by undergoing structural and residue changes in the MHV receptor, providing insight into this possible example of pathogen-driven evolution of a host receptor protein.

PubMed: 28035001
DOI: 10.1074/jbc.M116.764266

実験手法

X-RAY DIFFRACTION (3.1 Å)