5VSR

ABA-mimicking ligand AMF4 in complex with ABA receptor PYL2 and PP2C HAB1

Summary for 5VSR

• Entry DOI: 10.2210/pdb5vsr/pdb
• Related: 5VR7 5VRO 5VS5 5VSQ
• Descriptor: Abscisic acid receptor PYL2, Protein phosphatase 2C 16, N-(2-oxo-1-propyl-1,2,3,4-tetrahydroquinolin-6-yl)-1-(2,3,5,6-tetrafluoro-4-methylphenyl)methanesulfonamide, ... (5 entities in total)
• Functional Keywords: amf, aba, receptor, agonist, drought resistance, transferase
• Biological source: Arabidopsis thaliana (Mouse-ear cress); More
• Cellular location: Cytoplasm : O80992 Q9CAJ0
• Total number of polymer chains: 2
• Total formula weight: 58249.11

Authors

Cao, M.-J.,Zhang, Y.-L.,Liu, X.,Huang, H.,Zhou, X.E.,Wang, W.-L.,Zeng, A.,Zhao, C.-Z.,Si, T.,Du, J.-M.,Wu, W.-W.,Wang, F.-X.,Xu, H.X.,Zhu, J.-K. (deposition date: 2017-05-12, release date: 2017-11-15, Last modification date: 2024-03-13)

Primary citation

Cao, M.J.,Zhang, Y.L.,Liu, X.,Huang, H.,Zhou, X.E.,Wang, W.L.,Zeng, A.,Zhao, C.Z.,Si, T.,Du, J.,Wu, W.W.,Wang, F.X.,Xu, H.E.,Zhu, J.K.
Combining chemical and genetic approaches to increase drought resistance in plants.
Nat Commun, 8:1183-1183, 2017

PubMed Abstract: Drought stress is a major threat to crop production, but effective methods to mitigate the adverse effects of drought are not available. Here, we report that adding fluorine atoms in the benzyl ring of the abscisic acid (ABA) receptor agonist AM1 optimizes its binding to ABA receptors by increasing the number of hydrogen bonds between the compound and the surrounding amino acid residues in the receptor ligand-binding pocket. The new chemicals, known as AMFs, have long-lasting effects in promoting stomatal closure and inducing the expression of stress-responsive genes. Application of AMFs or transgenic overexpression of the receptor PYL2 in Arabidopsis and soybean plants confers increased drought resistance. The greatest increase in drought resistance is achieved when AMFs are applied to the PYL2-overexpression transgenic plants. Our results demonstrate that the combining of potent chemicals with transgenic overexpression of an ABA receptor is very effective in helping plants combat drought stress.

PubMed: 29084945
DOI: 10.1038/s41467-017-01239-3

Experimental method

X-RAY DIFFRACTION (2.618 Å)