5VQB

Crystal structure of rifampin monooxygenase from Streptomyces venezuelae, complex with FAD

5VQB の概要

• エントリーDOI: 10.2210/pdb5vqb/pdb
• 関連するPDBエントリー: 6BRD
• 分子名称: Rifampin monooxygenase, FLAVIN-ADENINE DINUCLEOTIDE, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: rifampin, rifamycin, antibiotic resistance, monooxygenase, oxidoreductase, fad, flavin adenine dinucleotide, structural genomics, csgid, center for structural genomics of infectious diseases, niaid, national institute of allergy and infectious disease
• 由来する生物種: Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 160143.30

構造登録者

Cox, G.,Kelso, J.,Stogios, P.J.,Savchenko, A.,Anderson, W.F.,Wright, G.D.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2017-05-08, 公開日: 2017-08-16, 最終更新日: 2023-10-04)

主引用文献

Koteva, K.,Cox, G.,Kelso, J.K.,Surette, M.D.,Zubyk, H.L.,Ejim, L.,Stogios, P.,Savchenko, A.,Sorensen, D.,Wright, G.D.
Rox, a Rifamycin Resistance Enzyme with an Unprecedented Mechanism of Action.
Cell Chem Biol, 25:403-412.e5, 2018

PubMed Abstract: Rifamycin monooxygenases (Rox) are present in a variety of environmental bacteria and are associated with decomposition of the clinically utilized antibiotic rifampin. Here we report the structure and function of a drug-inducible rox gene from Streptomyces venezuelae, which encodes a class A flavoprotein monooxygenase that inactivates a broad range of rifamycin antibiotics. Our findings describe a mechanism of rifamycin inactivation initiated by monooxygenation of the 2-position of the naphthyl group, which subsequently results in ring opening and linearization of the antibiotic. The result is an antibiotic that no longer adopts the basket-like structure essential for binding to the RNA exit tunnel of the target RpoB, thereby providing the molecular logic of resistance. This unique mechanism of enzymatic inactivation underpins the broad spectrum of rifamycin resistance mediated by Rox enzymes and presents a new antibiotic resistance mechanism not yet seen in microbial antibiotic detoxification.

PubMed: 29398560
DOI: 10.1016/j.chembiol.2018.01.009

実験手法

X-RAY DIFFRACTION (3.391 Å)