5VPE

Transcription factor FosB/JunD bZIP domain in complex with cognate DNA, type-I crystal

5VPE の概要

• エントリーDOI: 10.2210/pdb5vpe/pdb
• 関連するPDBエントリー: 5VPA 5VPB 5VPC 5VPD 5VPF
• 分子名称: Protein fosB, Transcription factor jun-D, DNA (5'-D(*CP*GP*TP*CP*GP*GP*TP*GP*AP*CP*TP*CP*AP*CP*CP*GP*AP*CP*G)-3'), ... (9 entities in total)
• 機能のキーワード: activator protein-1, basic leucine zipper, bzip, fos, jun, transcription factor, dna-binding protein, redox switch, coiled-coil, transcription-dna complex, transcription/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 56892.56

構造登録者

Yin, Z.,Machius, M.,Rudenko, G. (登録日: 2017-05-04, 公開日: 2017-09-06, 最終更新日: 2023-10-04)

主引用文献

Yin, Z.,Machius, M.,Nestler, E.J.,Rudenko, G.
Activator Protein-1: redox switch controlling structure and DNA-binding.
Nucleic Acids Res., 45:11425-11436, 2017

PubMed Abstract: The transcription factor, activator protein-1 (AP-1), binds to cognate DNA under redox control; yet, the underlying mechanism has remained enigmatic. A series of crystal structures of the AP-1 FosB/JunD bZIP domains reveal ordered DNA-binding regions in both FosB and JunD even in absence DNA. However, while JunD is competent to bind DNA, the FosB bZIP domain must undergo a large conformational rearrangement that is controlled by a 'redox switch' centered on an inter-molecular disulfide bond. Solution studies confirm that FosB/JunD cannot undergo structural transition and bind DNA when the redox-switch is in the 'OFF' state, and show that the mid-point redox potential of the redox switch affords it sensitivity to cellular redox homeostasis. The molecular and structural studies presented here thus reveal the mechanism underlying redox-regulation of AP-1 Fos/Jun transcription factors and provide structural insight for therapeutic interventions targeting AP-1 proteins.

PubMed: 28981703
DOI: 10.1093/nar/gkx795

実験手法

X-RAY DIFFRACTION (2.053 Å)