5VO8

X-ray crystal structure of a bacterial reiterative transcription complex of pyrG promoter

5VO8 の概要

• エントリーDOI: 10.2210/pdb5vo8/pdb
• 関連するPDBエントリー: 5VOI
• 分子名称: DNA-directed RNA polymerase subunit alpha, PYROPHOSPHATE 2-, ZINC ION, ... (11 entities in total)
• 機能のキーワード: thermus thermophilus, rna polymerase, reiterative transcription, holoenzyme, transcription-dna-rna complex, transcription/dna/rna
• 由来する生物種: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 444996.40

構造登録者

Murakami, K.S.,Shin, Y.,Turnbough Jr, C.L.,Molodtsov, V. (登録日: 2017-05-02, 公開日: 2017-05-24, 最終更新日: 2024-12-25)

主引用文献

Murakami, K.S.,Shin, Y.,Turnbough, C.L.,Molodtsov, V.
X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway.
Proc. Natl. Acad. Sci. U.S.A., 114:8211-8216, 2017

PubMed Abstract: Reiterative transcription is a noncanonical form of RNA synthesis in which a nucleotide specified by a single base in the DNA template is repetitively added to the nascent transcript. Here we determined the crystal structure of an RNA polymerase, the bacterial enzyme from , engaged in reiterative transcription during transcription initiation at a promoter resembling the promoter of The structure reveals that the reiterative transcript detours from the dedicated RNA exit channel and extends toward the main channel of the enzyme, thereby allowing RNA extension without displacement of the promoter recognition σ-factor. Nascent transcripts containing reiteratively added G residues are eventually extended by nonreiterative transcription, revealing an atypical pathway for the formation of a transcription elongation complex.

PubMed: 28652344
DOI: 10.1073/pnas.1702741114

実験手法

X-RAY DIFFRACTION (3.3 Å)