5VIV

Crystal structure of monomeric near-infrared fluorescent protein miRFP670

5VIV の概要

• エントリーDOI: 10.2210/pdb5viv/pdb
• 関連するPDBエントリー: 5VIK 5VIQ
• 分子名称: monomeric near-infrared fluorescent protein miRFP670, 3-[5-[[(3~{R},4~{R})-3-ethenyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid, 3-[2-[[5-[(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl] methyl]-5-[[(3~{R},4~{R})-3-ethyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]pro panoic acid, ... (5 entities in total)
• 機能のキーワード: near-infrared fluorescent protein, biliverdin, phytochrome, rpbphp1, fluorescent protein
• 由来する生物種: Rhodopseudomonas palustris
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35781.15

構造登録者

Pletnev, S. (登録日: 2017-04-17, 公開日: 2017-06-21, 最終更新日: 2024-10-16)

主引用文献

Baloban, M.,Shcherbakova, D.M.,Pletnev, S.,Pletnev, V.Z.,Lagarias, J.C.,Verkhusha, V.V.
Designing brighter near-infrared fluorescent proteins: insights from structural and biochemical studies.
Chem Sci, 8:4546-4557, 2017

PubMed Abstract: Brighter near-infrared (NIR) fluorescent proteins (FPs) are required for multicolor microscopy and deep-tissue imaging. Here, we present structural and biochemical analyses of three monomeric, spectrally distinct phytochrome-based NIR FPs, termed miRFPs. The miRFPs are closely related and differ by only a few amino acids, which define their molecular brightness, brightness in mammalian cells, and spectral properties. We have identified the residues responsible for the spectral red-shift, revealed a new chromophore bound simultaneously to two cysteine residues in the PAS and GAF domains in blue-shifted NIR FPs, and uncovered the importance of amino acid residues in the N-terminus of NIR FPs for their molecular and cellular brightness. The novel chromophore covalently links the N-terminus of NIR FPs with their C-terminal GAF domain, forming a topologically closed knot in the structure, and also contributes to the increased brightness. Based on our studies, we suggest a strategy to develop spectrally distinct NIR FPs with enhanced brightness.

PubMed: 28936332
DOI: 10.1039/c7sc00855d

実験手法

X-RAY DIFFRACTION (1.33 Å)