5VIN

Crystal Structure of the R515Q missense variant of human PGM1

5VIN の概要

• エントリーDOI: 10.2210/pdb5vin/pdb
• 関連するPDBエントリー: 5EPC
• 分子名称: Phosphoglucomutase-1, SULFATE ION, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: phosphoglucomutase-1, pgm1, isomerase, phosphoryl transfer
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 130244.58

構造登録者

Stiers, K.M.,Beamer, L.J. (登録日: 2017-04-17, 公開日: 2018-06-27, 最終更新日: 2023-10-04)

主引用文献

Stiers, K.M.,Beamer, L.J.
A Hotspot for Disease-Associated Variants of Human PGM1 Is Associated with Impaired Ligand Binding and Loop Dynamics.
Structure, 26:1337-, 2018

PubMed Abstract: Human phosphoglucomutase 1 (PGM1) plays a central role in cellular glucose homeostasis, catalyzing the conversion of glucose 1-phosphate and glucose 6-phosphate. Recently, missense variants of this enzyme were identified as causing an inborn error of metabolism, PGM1 deficiency, with features of a glycogen storage disease and a congenital disorder of glycosylation. Previous studies of selected PGM1 variants have revealed various mechanisms for enzyme dysfunction, including regions of structural disorder and side-chain rearrangements within the active site. Here, we examine variants within a substrate-binding loop in domain 4 (D4) of PGM1 that cause extreme impairment of activity. Biochemical, structural, and computational studies demonstrate multiple detrimental impacts resulting from these variants, including loss of conserved ligand-binding interactions and reduced mobility of the D4 loop, due to perturbation of its conformational ensemble. These potentially synergistic effects make this conserved ligand-binding loop a hotspot for disease-related variants in PGM1 and related enzymes.

PubMed: 30122451
DOI: 10.1016/j.str.2018.07.005

実験手法

X-RAY DIFFRACTION (2.60004291837 Å)