5VIM

Crystal structure of the Zika virus NS5 methyltransferase.

5VIM の概要

• エントリーDOI: 10.2210/pdb5vim/pdb
• 分子名称: Methyltransferase, S-ADENOSYLMETHIONINE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: methyltransferase, zika virus, viral protein
• 由来する生物種: Zika virus (strain Mr 766) (ZIKV)
• 細胞内の位置: Host endoplasmic reticulum membrane ; Peripheral membrane protein ; Lumenal side : A0A192GPS6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 59986.54

構造登録者

Bukrejewska, M.,Derewenda, Z.S.,Derewenda, U. (登録日: 2017-04-17, 公開日: 2017-09-06, 最終更新日: 2023-10-04)

主引用文献

Bukrejewska, M.,Derewenda, U.,Radwanska, M.,Engel, D.A.,Derewenda, Z.S.
Crystal structures of the methyltransferase and helicase from the ZIKA 1947 MR766 Uganda strain.
Acta Crystallogr D Struct Biol, 73:767-774, 2017

PubMed Abstract: Two nonstructural proteins encoded by Zika virus strain MR766 RNA, a methyltransferase and a helicase, were crystallized and their structures were solved and refined at 2.10 and 2.01 Å resolution, respectively. The NS5 methyltransferase contains a bound S-adenosyl-L-methionine (SAM) co-substrate. The NS3 helicase is in the apo form. Comparison with published crystal structures of the helicase in the apo, nucleotide-bound and single-stranded RNA (ssRNA)-bound states suggests that binding of ssRNA to the helicase may occur through conformational selection rather than induced fit.

PubMed: 28876240
DOI: 10.1107/S2059798317010737

実験手法

X-RAY DIFFRACTION (2.1 Å)