5VE9

Structure of hACF7 EF1-EF2-GAR domains

5VE9 の概要

• エントリーDOI: 10.2210/pdb5ve9/pdb
• 分子名称: Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5, CALCIUM ION, ZINC ION, ... (5 entities in total)
• 機能のキーワード: spectraplakin, ef-hand, microtubule binding, gas2, protein binding
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 30454.58

構造登録者

Lane, T.R.,Slep, K.C. (登録日: 2017-04-04, 公開日: 2017-06-21, 最終更新日: 2024-03-06)

主引用文献

Lane, T.R.,Fuchs, E.,Slep, K.C.
Structure of the ACF7 EF-Hand-GAR Module and Delineation of Microtubule Binding Determinants.
Structure, 25:1130-1138.e6, 2017

PubMed Abstract: Spectraplakins are large molecules that cross-link F-actin and microtubules (MTs). Mutations in spectraplakins yield defective cell polarization, aberrant focal adhesion dynamics, and dystonia. We present the 2.8 Å crystal structure of the hACF7 EF1-EF2-GAR MT-binding module and delineate the GAR residues critical for MT binding. The EF1-EF2 and GAR domains are autonomous domains connected by a flexible linker. The EF1-EF2 domain is an EFβ-scaffold with two bound Ca ions that straddle an N-terminal α helix. The GAR domain has a unique α/β sandwich fold that coordinates Zn. While the EF1-EF2 domain is not sufficient for MT binding, the GAR domain is and likely enhances EF1-EF2-MT engagement. Residues in a conserved basic patch, distal to the GAR domain's Zn-binding site, mediate MT binding.

PubMed: 28602822
DOI: 10.1016/j.str.2017.05.006

実験手法

X-RAY DIFFRACTION (2.795 Å)