5VAS

Pekin duck egg lysozyme isoform III (DEL-III), orthorhombic form

5VAS の概要

• エントリーDOI: 10.2210/pdb5vas/pdb
• 関連するPDBエントリー: 5V8G 5V92 5V94
• 分子名称: Lysozyme, PHOSPHATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: duck lysozyme, glycosyl hydrolase, n-acetylmuramidase, hydrolase
• 由来する生物種: Anas platyrhynchos (Mallard, Pekin duck)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30002.60

構造登録者

Christie, M.,Christ, D.,Langley, D.B. (登録日: 2017-03-27, 公開日: 2018-04-18, 最終更新日: 2024-11-06)

主引用文献

Langley, D.B.,Crossett, B.,Schofield, P.,Jackson, J.,Zeraati, M.,Maltby, D.,Christie, M.,Burnett, D.,Brink, R.,Goodnow, C.,Christ, D.
Structural basis of antigen recognition: crystal structure of duck egg lysozyme.
Acta Crystallogr D Struct Biol, 73:910-920, 2017

PubMed Abstract: Duck egg lysozyme (DEL) is a widely used model antigen owing to its capacity to bind with differential affinity to anti-chicken egg lysozyme antibodies. However, no structures of DEL have so far been reported, and the situation had been complicated by the presence of multiple isoforms and conflicting reports of primary sequence. Here, the structures of two DEL isoforms from the eggs of the commonly used Pekin duck (Anas platyrhynchos) are reported. Using structural analyses in combination with mass spectrometry, non-ambiguous DEL primary sequences are reported. Furthermore, the structures and sequences determined here enable rationalization of the binding affinity of DEL for well documented landmark anti-lysozyme antibodies.

PubMed: 29095163
DOI: 10.1107/S2059798317013730

実験手法

X-RAY DIFFRACTION (1.4 Å)