5V7V

Cryo-EM structure of ERAD-associated E3 ubiquitin-protein ligase component HRD3

5V7V の概要

• エントリーDOI: 10.2210/pdb5v7v/pdb
• 関連するPDBエントリー: 5V6P
• EMDBエントリー: 8637 8638 8639 8642
• 分子名称: ERAD-associated E3 ubiquitin-protein ligase component HRD3, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: hrd3 erad, protein transport
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 95595.86

構造登録者

Mi, W.,Schoebel, S.,Stein, A.,Rapoport, T.A.,Liao, M. (登録日: 2017-03-20, 公開日: 2017-08-16, 最終更新日: 2025-06-04)

主引用文献

Schoebel, S.,Mi, W.,Stein, A.,Ovchinnikov, S.,Pavlovicz, R.,DiMaio, F.,Baker, D.,Chambers, M.G.,Su, H.,Li, D.,Rapoport, T.A.,Liao, M.
Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3.
Nature, 548:352-355, 2017

PubMed Abstract: Misfolded endoplasmic reticulum proteins are retro-translocated through the membrane into the cytosol, where they are poly-ubiquitinated, extracted from the membrane, and degraded by the proteasome-a pathway termed endoplasmic reticulum-associated protein degradation (ERAD). Proteins with misfolded domains in the endoplasmic reticulum lumen or membrane are discarded through the ERAD-L and ERAD-M pathways, respectively. In Saccharomyces cerevisiae, both pathways require the ubiquitin ligase Hrd1, a multi-spanning membrane protein with a cytosolic RING finger domain. Hrd1 is the crucial membrane component for retro-translocation, but it is unclear whether it forms a protein-conducting channel. Here we present a cryo-electron microscopy structure of S. cerevisiae Hrd1 in complex with its endoplasmic reticulum luminal binding partner, Hrd3. Hrd1 forms a dimer within the membrane with one or two Hrd3 molecules associated at its luminal side. Each Hrd1 molecule has eight transmembrane segments, five of which form an aqueous cavity extending from the cytosol almost to the endoplasmic reticulum lumen, while a segment of the neighbouring Hrd1 molecule forms a lateral seal. The aqueous cavity and lateral gate are reminiscent of features of protein-conducting conduits that facilitate polypeptide movement in the opposite direction-from the cytosol into or across membranes. Our results suggest that Hrd1 forms a retro-translocation channel for the movement of misfolded polypeptides through the endoplasmic reticulum membrane.

PubMed: 28682307
DOI: 10.1038/nature23314

実験手法

ELECTRON MICROSCOPY (3.9 Å)