5V5X

Protocadherin gammaB7 EC3-6 cis-dimer structure

5V5X の概要

• エントリーDOI: 10.2210/pdb5v5x/pdb
• 分子名称: MCG133388, isoform CRA_y, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: cell adhesion
• 由来する生物種: Mus musculus (Mouse)
• 細胞内の位置: Cell membrane ; Single-pass type I membrane protein : Q91XX3
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 201016.69

構造登録者

Goodman, K.M.,Mannepalli, S.,Bahna, F.,Honig, B.,Shapiro, L. (登録日: 2017-03-15, 公開日: 2017-11-01, 最終更新日: 2024-11-20)

主引用文献

Goodman, K.M.,Rubinstein, R.,Dan, H.,Bahna, F.,Mannepalli, S.,Ahlsen, G.,Aye Thu, C.,Sampogna, R.V.,Maniatis, T.,Honig, B.,Shapiro, L.
Protocadherin cis-dimer architecture and recognition unit diversity.
Proc. Natl. Acad. Sci. U.S.A., 114:E9829-E9837, 2017

PubMed Abstract: Clustered protocadherins (Pcdhs) mediate numerous neural patterning functions, including neuronal self-recognition and non-self-discrimination to direct self-avoidance among vertebrate neurons. Individual neurons stochastically express a subset of Pcdh isoforms, which assemble to form a stochastic repertoire of -dimers. We describe the structure of a PcdhγB7 -homodimer, which includes the membrane-proximal extracellular cadherin domains EC5 and EC6. The structure is asymmetric with one molecule contributing interface surface from both EC5 and EC6, and the other only from EC6. Structural and sequence analyses suggest that all Pcdh isoforms will dimerize through this interface. Site-directed mutants at this interface interfere with both Pcdh -dimerization and cell surface transport. The structure explains the known restrictions of -interactions of some Pcdh isoforms, including α-Pcdhs, which cannot form homodimers. The asymmetry of the interface approximately doubles the size of the recognition repertoire, and restrictions on -interactions among Pcdh isoforms define the limits of the Pcdh recognition unit repertoire.

PubMed: 29087338
DOI: 10.1073/pnas.1713449114

実験手法

X-RAY DIFFRACTION (3.5 Å)