5UZY

Crystal structure of N97A mutant of human macrophage migration inhibitory factor

5UZY の概要

• エントリーDOI: 10.2210/pdb5uzy/pdb
• 分子名称: Macrophage migration inhibitory factor, SULFATE ION, ISOPROPYL ALCOHOL, ... (5 entities in total)
• 機能のキーワード: isomerase, trimeric, mutation
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 37592.72

構造登録者

Pantouris, G.,Lolis, E. (登録日: 2017-02-27, 公開日: 2018-02-28, 最終更新日: 2023-10-04)

主引用文献

Pantouris, G.,Ho, J.,Shah, D.,Syed, M.A.,Leng, L.,Bhandari, V.,Bucala, R.,Batista, V.S.,Loria, J.P.,Lolis, E.J.
Nanosecond Dynamics Regulate the MIF-Induced Activity of CD74.
Angew. Chem. Int. Ed. Engl., 57:7116-7119, 2018

PubMed Abstract: Macrophage migration inhibitory factor (MIF) activates CD74, which leads to severe disorders including inflammation, autoimmune diseases and cancer under pathological conditions. Molecular dynamics (MD) simulations up to one microsecond revealed dynamical correlation between a residue located at the opening of one end of the MIF solvent channel, previously thought to be a consequence of homotrimerization, and residues in a distal region responsible for CD74 activation. Experiments verified the allosteric regulatory site and identified a pathway to this site via the MIF β-strands. The reported findings provide fundamental insights on a dynamic mechanism that controls the MIF-induced activation of CD74.

PubMed: 29669180
DOI: 10.1002/anie.201803191

実験手法

X-RAY DIFFRACTION (1.71 Å)