5UTT

SrtA sortase from Actinomyces oris

5UTT の概要

• エントリーDOI: 10.2210/pdb5utt/pdb
• 分子名称: Sortase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: structural genomics, center for structural genomics of infectious diseases, csgid, hydrolase
• 由来する生物種: Actinomyces oris
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 84134.54

構造登録者

Osipiuk, J.,Ma, X.,Ton-That, H.,Anderson, W.F.,Joachimiak, A.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2017-02-15, 公開日: 2017-03-01, 最終更新日: 2023-10-04)

主引用文献

Chang, C.,Wu, C.,Osipiuk, J.,Siegel, S.D.,Zhu, S.,Liu, X.,Joachimiak, A.,Clubb, R.T.,Das, A.,Ton-That, H.
Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes.
Proc.Natl.Acad.Sci.USA, 116:18041-18049, 2019

PubMed Abstract: Assembly of pili on the gram-positive bacterial cell wall involves 2 conserved transpeptidase enzymes named sortases: One for polymerization of pilin subunits and another for anchoring pili to peptidoglycan. How this machine controls pilus length and whether pilus length is critical for cell-to-cell interactions remain unknown. We report here in , a key colonizer in the development of oral biofilms, that genetic disruption of its housekeeping sortase SrtA generates exceedingly long pili, catalyzed by its pilus-specific sortase SrtC2 that possesses both pilus polymerization and cell wall anchoring functions. Remarkably, the deficient mutant fails to mediate interspecies interactions, or coaggregation, even though the coaggregation factor CafA is present at the pilus tip. Increasing ectopic expression of in the mutant progressively shortens pilus length and restores coaggregation accordingly, while elevated levels of shaft pilins and SrtC2 produce long pili and block coaggregation by SrtA bacteria. With structural studies, we uncovered 2 key structural elements in SrtA that partake in recognition of pilin substrates and regulate pilus length by inducing the capture and transfer of pilus polymers to the cell wall. Evidently, coaggregation requires proper positioning of the tip adhesin CafA via modulation of pilus length by the housekeeping sortase SrtA.

PubMed: 31427528
DOI: 10.1073/pnas.1907733116

実験手法

X-RAY DIFFRACTION (1.7 Å)