5UKG

Crystal Structure of the genetically encoded calcium indicator K-GECO

5UKG の概要

• エントリーDOI: 10.2210/pdb5ukg/pdb
• 分子名称: K-GECO, CALCIUM ION (3 entities in total)
• 機能のキーワード: calcium indicator fluorescent protein, fluorescent protein
• 由来する生物種: Entacmaea quadricolor
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93647.61

構造登録者

Schreiter, E.R. (登録日: 2017-01-22, 公開日: 2018-02-07, 最終更新日: 2026-03-18)

主引用文献

Shen, Y.,Dana, H.,Abdelfattah, A.S.,Patel, R.,Shea, J.,Molina, R.S.,Rawal, B.,Rancic, V.,Chang, Y.F.,Wu, L.,Chen, Y.,Qian, Y.,Wiens, M.D.,Hambleton, N.,Ballanyi, K.,Hughes, T.E.,Drobizhev, M.,Kim, D.S.,Koyama, M.,Schreiter, E.R.,Campbell, R.E.
A genetically encoded Ca2+indicator based on circularly permutated sea anemone red fluorescent protein eqFP578.
BMC Biol., 16:9-9, 2018

PubMed Abstract: Genetically encoded calcium ion (Ca) indicators (GECIs) are indispensable tools for measuring Ca dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation. Longer wavelength light is associated with decreased phototoxicity and deeper penetration through tissue. Red GECI can also enable multicolor visualization with blue- or cyan-excitable fluorophores.

PubMed: 29338710
DOI: 10.1186/s12915-018-0480-0

実験手法

X-RAY DIFFRACTION (2.36 Å)