5UJ6

Crystal Structure of Bacteroides Uniformis beta-glucuronidase

5UJ6 の概要

• エントリーDOI: 10.2210/pdb5uj6/pdb
• 分子名称: Glycosyl hydrolases family 2, sugar binding domain protein, GLYCEROL, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: glucuronic-acid carbohydrate, hydrolase
• 由来する生物種: Bacteroides uniformis str. 3978 T3 ii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 201741.34

構造登録者

Walton, W.G.,Redinbo, M.R. (登録日: 2017-01-17, 公開日: 2017-06-28, 最終更新日: 2024-03-06)

主引用文献

Pollet, R.M.,D'Agostino, E.H.,Walton, W.G.,Xu, Y.,Little, M.S.,Biernat, K.A.,Pellock, S.J.,Patterson, L.M.,Creekmore, B.C.,Isenberg, H.N.,Bahethi, R.R.,Bhatt, A.P.,Liu, J.,Gharaibeh, R.Z.,Redinbo, M.R.
An Atlas of beta-Glucuronidases in the Human Intestinal Microbiome.
Structure, 25:967-977.e5, 2017

PubMed Abstract: Microbiome-encoded β-glucuronidase (GUS) enzymes play important roles in human health by metabolizing drugs in the gastrointestinal (GI) tract. The numbers, types, and diversity of these proteins in the human GI microbiome, however, remain undefined. We present an atlas of GUS enzymes comprehensive for the Human Microbiome Project GI database. We identify 3,013 total and 279 unique microbiome-encoded GUS proteins clustered into six unique structural categories. We assign their taxonomy, assess cellular localization, reveal the inter-individual variability within the 139 individuals sampled, and discover 112 novel microbial GUS enzymes. A representative in vitro panel of the most common GUS proteins by read abundances highlights structural and functional variabilities within the family, including their differential processing of smaller glucuronides and larger carbohydrates. These data provide a sequencing-to-molecular roadmap for examining microbiome-encoded enzymes essential to human health.

PubMed: 28578872
DOI: 10.1016/j.str.2017.05.003

実験手法

X-RAY DIFFRACTION (1.9 Å)