5UJ2

Crystal structure of HCV NS5B genotype 2A JFH-1 isolate with S15G E86Q E87Q C223H V321I mutations and Delta8 neta hairpoin loop deletion in complex with GS-639476 (diphsohate version of GS-9813), Mn2+ and symmetrical primer template 5'-AUAAAUUU

Summary for 5UJ2

• Entry DOI: 10.2210/pdb5uj2/pdb
• Related: 4WTD 4WTG
• Descriptor: RNA (5'-R(*AP*UP*AP*AP*AP*UP*UP*U)-3'), Genome polyprotein, MANGANESE (II) ION, ... (6 entities in total)
• Functional Keywords: hcv, viral, ns5b, rdrp, resistance mutation, sofosbuvir, sovaldi, virunon, gs7977-dp, gs-639476, template, primer, immune system-rna complex, immune system/rna
• Biological source: Escherichia coli; More
• Cellular location: Virion : R9TEU1
• Total number of polymer chains: 3
• Total formula weight: 69497.88

Authors

Edwards, T.E.,Fox III, D.,Appleby, T.C.,Murakami, E.,Rey, A.,McGrath, M.E. (deposition date: 2017-01-16, release date: 2017-03-22, Last modification date: 2023-10-04)

• Primary citation: Kirschberg, T.A.,Metobo, S.,Clarke, M.O.,Aktoudianakis, V.,Babusis, D.,Barauskas, O.,Birkus, G.,Butler, T.,Byun, D.,Chin, G.,Doerffler, E.,Edwards, T.E.,Fenaux, M.,Lee, R.,Lew, W.,Mish, M.R.,Murakami, E.,Park, Y.,Squires, N.H.,Tirunagari, N.,Wang, T.,Whitcomb, M.,Xu, J.,Yang, H.,Ye, H.,Zhang, L.,Appleby, T.C.,Feng, J.Y.,Ray, A.S.,Cho, A.,Kim, C.U.; Discovery of a 2'-fluoro-2'-C-methyl C-nucleotide HCV polymerase inhibitor and a phosphoramidate prodrug with favorable properties.; Bioorg. Med. Chem. Lett., 27:1840-1847, 2017; PubMed: 28274633; DOI: 10.1016/j.bmcl.2017.02.037

Experimental method

X-RAY DIFFRACTION (2.9 Å)