5UIX

Crystal Structure of the DH576 CD4bs Fab (unliganded) from the RV305 HIV Vaccine Trial

5UIX の概要

• エントリーDOI: 10.2210/pdb5uix/pdb
• EMDBエントリー: 8573
• 分子名称: DH576 Fab heavy chain, DH576 Fab light chain (3 entities in total)
• 機能のキーワード: fab fragment, hiv-1, antibody, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48318.93

構造登録者

Fera, D.,Harrison, S.C. (登録日: 2017-01-15, 公開日: 2017-03-08, 最終更新日: 2024-10-30)

主引用文献

Easterhoff, D.,Moody, M.A.,Fera, D.,Cheng, H.,Ackerman, M.,Wiehe, K.,Saunders, K.O.,Pollara, J.,Vandergrift, N.,Parks, R.,Kim, J.,Michael, N.L.,O'Connell, R.J.,Excler, J.L.,Robb, M.L.,Vasan, S.,Rerks-Ngarm, S.,Kaewkungwal, J.,Pitisuttithum, P.,Nitayaphan, S.,Sinangil, F.,Tartaglia, J.,Phogat, S.,Kepler, T.B.,Alam, S.M.,Liao, H.X.,Ferrari, G.,Seaman, M.S.,Montefiori, D.C.,Tomaras, G.D.,Harrison, S.C.,Haynes, B.F.
Boosting of HIV envelope CD4 binding site antibodies with long variable heavy third complementarity determining region in the randomized double blind RV305 HIV-1 vaccine trial.
PLoS Pathog., 13:e1006182-e1006182, 2017

PubMed Abstract: The canary pox vector and gp120 vaccine (ALVAC-HIV and AIDSVAX B/E gp120) in the RV144 HIV-1 vaccine trial conferred an estimated 31% vaccine efficacy. Although the vaccine Env AE.A244 gp120 is antigenic for the unmutated common ancestor of V1V2 broadly neutralizing antibody (bnAbs), no plasma bnAb activity was induced. The RV305 (NCT01435135) HIV-1 clinical trial was a placebo-controlled randomized double-blinded study that assessed the safety and efficacy of vaccine boosting on B cell repertoires. HIV-1-uninfected RV144 vaccine recipients were reimmunized 6-8 years later with AIDSVAX B/E gp120 alone, ALVAC-HIV alone, or a combination of ALVAC-HIV and AIDSVAX B/E gp120 in the RV305 trial. Env-specific post-RV144 and RV305 boost memory B cell VH mutation frequencies increased from 2.9% post-RV144 to 6.7% post-RV305. The vaccine was well tolerated with no adverse events reports. While post-boost plasma did not have bnAb activity, the vaccine boosts expanded a pool of envelope CD4 binding site (bs)-reactive memory B cells with long third heavy chain complementarity determining regions (HCDR3) whose germline precursors and affinity matured B cell clonal lineage members neutralized the HIV-1 CRF01 AE tier 2 (difficult to neutralize) primary isolate, CNE8. Electron microscopy of two of these antibodies bound with near-native gp140 trimers showed that they recognized an open conformation of the Env trimer. Although late boosting of RV144 vaccinees expanded a novel pool of neutralizing B cell clonal lineages, we hypothesize that boosts with stably closed trimers would be necessary to elicit antibodies with greater breadth of tier 2 HIV-1 strains.

PubMed: 28235027
DOI: 10.1371/journal.ppat.1006182

実験手法

X-RAY DIFFRACTION (2.501 Å)