5UHY

A Human Antibody Against Zika Virus Crosslinks the E Protein to Prevent Infection

5UHY の概要

• エントリーDOI: 10.2210/pdb5uhy/pdb
• EMDBエントリー: 8548
• 分子名称: ZV67 Fab chain 1, ZV67 Fab chain 2, envelope protein (3 entities in total)
• 機能のキーワード: flavivirus, zika, human antibody, therapeutic, virus-immune system complex, virus/immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 222837.55

構造登録者

Hasan, S.S.,Miller, A.,Sapparapu, G.,Fernandez, E.,Klose, T.,Long, F.,Fokine, A.,Porta, J.C.,Jiang, W.,Diamond, M.S.,Crowe Jr., J.E.,Kuhn, R.J.,Rossmann, M.G. (登録日: 2017-01-12, 公開日: 2017-03-29, 最終更新日: 2024-11-13)

主引用文献

Hasan, S.S.,Miller, A.,Sapparapu, G.,Fernandez, E.,Klose, T.,Long, F.,Fokine, A.,Porta, J.C.,Jiang, W.,Diamond, M.S.,Crowe, J.E.,Kuhn, R.J.,Rossmann, M.G.
A human antibody against Zika virus crosslinks the E protein to prevent infection.
Nat Commun, 8:14722-14722, 2017

PubMed Abstract: The recent Zika virus (ZIKV) epidemic has been linked to unusual and severe clinical manifestations including microcephaly in fetuses of infected pregnant women and Guillian-Barré syndrome in adults. Neutralizing antibodies present a possible therapeutic approach to prevent and control ZIKV infection. Here we present a 6.2 Å resolution three-dimensional cryo-electron microscopy (cryoEM) structure of an infectious ZIKV (strain H/PF/2013, French Polynesia) in complex with the Fab fragment of a highly therapeutic and neutralizing human monoclonal antibody, ZIKV-117. The antibody had been shown to prevent fetal infection and demise in mice. The structure shows that ZIKV-117 Fabs cross-link the monomers within the surface E glycoprotein dimers as well as between neighbouring dimers, thus preventing the reorganization of E protein monomers into fusogenic trimers in the acidic environment of endosomes.

PubMed: 28300075
DOI: 10.1038/ncomms14722

実験手法

ELECTRON MICROSCOPY (6.2 Å)