5UCY

Cryo-EM map of protofilament of microtubule doublet

5UCY の概要

• エントリーDOI: 10.2210/pdb5ucy/pdb
• 関連するPDBエントリー: 5UBQ
• EMDBエントリー: 8528 8532 8537 8539
• 分子名称: Tubulin alpha chain, Tubulin beta chain, GUANOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: cilia, doublet, protofilament, tubulin, structural protein
• 由来する生物種: Tetrahymena thermophila; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 97788.14

構造登録者

Ichikawa, M.,Liu, D.,Kastritis, P.L.,Basu, K.,Bui, K.H. (登録日: 2016-12-22, 公開日: 2017-05-10, 最終更新日: 2024-12-25)

主引用文献

Ichikawa, M.,Liu, D.,Kastritis, P.L.,Basu, K.,Hsu, T.C.,Yang, S.,Bui, K.H.
Subnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins.
Nat Commun, 8:15035-15035, 2017

PubMed Abstract: Cilia are ubiquitous, hair-like appendages found in eukaryotic cells that carry out functions of cell motility and sensory reception. Cilia contain an intriguing cytoskeletal structure, termed the axoneme that consists of nine doublet microtubules radially interlinked and longitudinally organized in multiple specific repeat units. Little is known, however, about how the axoneme allows cilia to be both actively bendable and sturdy or how it is assembled. To answer these questions, we used cryo-electron microscopy to structurally analyse several of the repeating units of the doublet at sub-nanometre resolution. This structural detail enables us to unambiguously assign α- and β-tubulins in the doublet microtubule lattice. Our study demonstrates the existence of an inner sheath composed of different kinds of microtubule inner proteins inside the doublet that likely stabilizes the structure and facilitates the specific building of the B-tubule.

PubMed: 28462916
DOI: 10.1038/ncomms15035

実験手法

ELECTRON MICROSCOPY (4.6 Å)