5UBP

TREX2 M-region

5UBP の概要

• エントリーDOI: 10.2210/pdb5ubp/pdb
• 分子名称: Leucine permease transcriptional regulator, Nuclear mRNA export protein THP1, 26S proteasome complex subunit SEM1, ... (4 entities in total)
• 機能のキーワード: trex2 complex; tpr repeats, transcription
• 由来する生物種: Saccharomyces cerevisiae RM11-1a; 詳細
• 細胞内の位置: Nucleus envelope : Q08231
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 119295.10

構造登録者

Stewart, M.,Gordon, J. (登録日: 2016-12-21, 公開日: 2017-04-05, 最終更新日: 2024-11-06)

主引用文献

Gordon, J.M.B.,Aibara, S.,Stewart, M.
Structure of the Sac3 RNA-binding M-region in the Saccharomyces cerevisiae TREX-2 complex.
Nucleic Acids Res., 45:5577-5585, 2017

PubMed Abstract: Transcription-export complex 2 (TREX-2, or THSC) facilitates localization of actively transcribing genes such as GAL1 to the nuclear periphery, contributes to the generation of export-competent mRNPs and influences gene expression through interactions with Mediator. TREX-2 is based on a Sac3 scaffold to which Thp1, Sem1, Cdc31 and Sus1 bind and consists of three modules: the N-region (Sac3∼1-100), which binds mRNA export factor Mex67:Mtr2; the M-region, in which Thp1 and Sem1 bind to Sac3∼100-550; and the CID region in which Cdc31 and two Sus1 chains bind to Sac3∼720-805. Although the M-region of Sac3 was originally thought to encompass residues ∼250-550, we report here the 2.3Å resolution crystal structure of a complex containing Sac3 residues 60-550 that indicates that the TPR-like repeats of the M-region extend to residue 137 and that residues 90-125 form a novel loop that links Sac3 to Thp1. These new structural elements are important for growth and mRNA export in vivo. Although deleting Sac3 residues 1-90 produced a wild-type phenotype, deletion of the loop as well generated growth defects at 37°C, whereas the deletion of residues 1-250 impaired mRNA export and also generated longer lag times when glucose or raffinose was replaced by galactose as the carbon source.

PubMed: 28334829
DOI: 10.1093/nar/gkx158

実験手法

X-RAY DIFFRACTION (2.3 Å)