5U4R

Crystal structure of the broadly neutralizing Influenza A antibody VRC 315 53-1A09 Fab.

Summary for 5U4R

• Entry DOI: 10.2210/pdb5u4r/pdb
• Descriptor: VRC 315 53-1A09 Fab Heavy chain, VRC 315 53-1A09 Fab Light chain (3 entities in total)
• Functional Keywords: immune system, antibody, influenza, human vaccine trial
• Biological source: Homo sapiens; More
• Total number of polymer chains: 4
• Total formula weight: 97074.20

Authors

Joyce, M.G.,Andrews, S.F.,Mascola, J.R.,McDermott, A.B.,Kwong, P.D. (deposition date: 2016-12-05, release date: 2017-11-15, Last modification date: 2024-10-16)

Primary citation

Andrews, S.F.,Joyce, M.G.,Chambers, M.J.,Gillespie, R.A.,Kanekiyo, M.,Leung, K.,Yang, E.S.,Tsybovsky, Y.,Wheatley, A.K.,Crank, M.C.,Boyington, J.C.,Prabhakaran, M.S.,Narpala, S.R.,Chen, X.,Bailer, R.T.,Chen, G.,Coates, E.,Kwong, P.D.,Koup, R.A.,Mascola, J.R.,Graham, B.S.,Ledgerwood, J.E.,McDermott, A.B.
Preferential induction of cross-group influenza A hemagglutinin stem-specific memory B cells after H7N9 immunization in humans.
Sci Immunol, 2:-, 2017

PubMed Abstract: Antigenic drift and shift of influenza strains underscore the need for broadly protective influenza vaccines. One strategy is to design immunogens that elicit B cell responses against conserved epitopes on the hemagglutinin (HA) stem. To better understand the elicitation of HA stem-targeted B cells to group 1 and group 2 influenza subtypes, we compared the memory B cell response to group 2 H7N9 and group 1 H5N1 vaccines in humans. Upon H7N9 vaccination, almost half of the HA stem-specific response recognized the group 1 and group 2 subtypes, whereas the response to H5N1 was largely group 1-specific. Immunoglobulin repertoire analysis of HA-specific B cells indicated that the H7N9 and H5N1 vaccines induced genetically similar cross-group HA stem-binding B cells, albeit at a much higher frequency upon H7N9 vaccination. These data suggest that a group 2-based stem immunogen could prove more effective than a group 1 immunogen at eliciting broad cross-group protection in humans.

PubMed: 28783708
DOI: 10.1126/sciimmunol.aan2676

Experimental method

X-RAY DIFFRACTION (1.762 Å)