5U3N

Crystal Structure of DH511.12P Fab in Complex with HIV-1 gp41 MPER Peptide

5U3N の概要

• エントリーDOI: 10.2210/pdb5u3n/pdb
• 関連するPDBエントリー: 5U3J 5U3K 5U3L 5U3M 5U3O 5U3P
• 分子名称: DH511.12P Fab Heavy Chain, DH511.12P Fab Light Chain, gp41 MPER peptide, ... (4 entities in total)
• 機能のキーワード: hiv-1, neutralizing, antibody, gp41, mper, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 52809.45

構造登録者

Ofek, G.,Wu, L.,Lougheed, C.S.,Williams, L.D.,Nicely, N.I.,Haynes, B.F. (登録日: 2016-12-02, 公開日: 2017-02-15, 最終更新日: 2024-11-06)

主引用文献

Williams, L.D.,Ofek, G.,Schatzle, S.,McDaniel, J.R.,Lu, X.,Nicely, N.I.,Wu, L.,Lougheed, C.S.,Bradley, T.,Louder, M.K.,McKee, K.,Bailer, R.T.,O'Dell, S.,Georgiev, I.S.,Seaman, M.S.,Parks, R.J.,Marshall, D.J.,Anasti, K.,Yang, G.,Nie, X.,Tumba, N.L.,Wiehe, K.,Wagh, K.,Korber, B.,Kepler, T.B.,Munir Alam, S.,Morris, L.,Kamanga, G.,Cohen, M.S.,Bonsignori, M.,Xia, S.M.,Montefiori, D.C.,Kelsoe, G.,Gao, F.,Mascola, J.R.,Moody, M.A.,Saunders, K.O.,Liao, H.X.,Tomaras, G.D.,Georgiou, G.,Haynes, B.F.
Potent and broad HIV-neutralizing antibodies in memory B cells and plasma.
Sci Immunol, 2:-, 2017

PubMed Abstract: Induction of broadly neutralizing antibodies (bnAbs) is a goal of HIV-1 vaccine development. Antibody 10E8, reactive with the distal portion of the membrane-proximal external region (MPER) of HIV-1 gp41, is broadly neutralizing. However, the ontogeny of distal MPER antibodies and the relationship of memory B cell to plasma bnAbs are poorly understood. HIV-1-specific memory B cell flow sorting and proteomic identification of anti-MPER plasma antibodies from an HIV-1-infected individual were used to isolate broadly neutralizing distal MPER bnAbs of the same B cell clonal lineage. Structural analysis demonstrated that antibodies from memory B cells and plasma recognized the envelope gp41 bnAb epitope in a distinct orientation compared with other distal MPER bnAbs. The unmutated common ancestor of this distal MPER bnAb was autoreactive, suggesting lineage immune tolerance control. Construction of chimeric antibodies of memory B cell and plasma antibodies yielded a bnAb that potently neutralized most HIV-1 strains.

PubMed: 28783671
DOI: 10.1126/sciimmunol.aal2200

実験手法

X-RAY DIFFRACTION (2 Å)