5TYH

PglD from Campylobacter jejuni NCTC 11168 in complex with 5-(2-furanyl)-1H-pyrazole-3-carboxylic acid

5TYH の概要

• エントリーDOI: 10.2210/pdb5tyh/pdb
• 分子名称: UDP-N-acetylbacillosamine N-acetyltransferase, 3-(furan-2-yl)-1H-pyrazole-5-carboxylic acid (3 entities in total)
• 機能のキーワード: acetyltransferase bacterial n-glycan biosynthesis inhibitor fragment-based discovery, transferase, transferase-transferase inhibitor complex, transferase/transferase inhibitor
• 由来する生物種: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21746.25

構造登録者

Morrison, J.P.,Imperiali, B. (登録日: 2016-11-20, 公開日: 2017-04-12, 最終更新日: 2023-10-04)

主引用文献

De Schutter, J.W.,Morrison, J.P.,Morrison, M.J.,Ciulli, A.,Imperiali, B.
Targeting Bacillosamine Biosynthesis in Bacterial Pathogens: Development of Inhibitors to a Bacterial Amino-Sugar Acetyltransferase from Campylobacter jejuni.
J. Med. Chem., 60:2099-2118, 2017

PubMed Abstract: The glycoproteins of selected microbial pathogens often include highly modified carbohydrates such as 2,4-diacetamidobacillosamine (diNAcBac). These glycoconjugates are involved in host-cell interactions and may be associated with the virulence of medically significant Gram-negative bacteria. In light of genetic studies demonstrating the attenuated virulence of bacterial strains in which modified carbohydrate biosynthesis enzymes have been knocked out, we are developing small molecule inhibitors of selected enzymes as tools to evaluate whether such compounds modulate virulence. We performed fragment-based and high-throughput screens against an amino-sugar acetyltransferase enzyme, PglD, involved in biosynthesis of UDP-diNAcBac in Campylobacter jejuni. Herein we report optimization of the hits into potent small molecule inhibitors (IC < 300 nM). Biophysical characterization shows that the best inhibitors are competitive with acetyl coenzyme A and an X-ray cocrystal structure reveals that binding is biased toward occupation of the adenine subpocket of the AcCoA binding site by an aromatic ring.

PubMed: 28182413
DOI: 10.1021/acs.jmedchem.6b01869

実験手法

X-RAY DIFFRACTION (2.1 Å)