5TW5

Structure of mouse CD1d with bound glycosphingolipid JJ112

5TW5 の概要

• エントリーDOI: 10.2210/pdb5tw5/pdb
• 関連するPDBエントリー: 5TW2
• 分子名称: Antigen-presenting glycoprotein CD1d1, Beta-2-microglobulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: mhc fold, antigen-presentation, glycolipid, t cells, immune system-inhibitor complex, immune system/inhibitor
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45823.86

構造登録者

Zajonc, D.M.,Wang, J. (登録日: 2016-11-11, 公開日: 2017-09-20, 最終更新日: 2024-10-16)

主引用文献

Guillaume, J.,Wang, J.,Janssens, J.,Remesh, S.G.,Risseeuw, M.D.P.,Decruy, T.,Froeyen, M.,Elewaut, D.,Zajonc, D.M.,Calenbergh, S.V.
Galactosylsphingamides: new alpha-GalCer analogues to probe the F'-pocket of CD1d.
Sci Rep, 7:4276-4276, 2017

PubMed Abstract: Invariant Natural Killer T-cells (iNKT-cells) are an attractive target for immune response modulation, as upon CD1d-mediated stimulation with KRN7000, a synthetic α-galactosylceramide, they produce a vast amount of cytokines. Here we present a synthesis that allows swift modification of the phytosphingosine side chain by amidation of an advanced methyl ester precursor. The resulting KRN7000 derivatives, termed α-galactosylsphingamides, were evaluated for their capacity to stimulate iNKT-cells. While introduction of the amide-motif in the phytosphingosine chain is tolerated for CD1d binding and TCR recognition, the studied α-galactosylsphingamides showed compromised antigenic properties.

PubMed: 28655912
DOI: 10.1038/s41598-017-04461-7

実験手法

X-RAY DIFFRACTION (1.85 Å)