5TMH

Structure of Zika virus NS5

5TMH の概要

• エントリーDOI: 10.2210/pdb5tmh/pdb
• 分子名称: Polyprotein, S-ADENOSYL-L-HOMOCYSTEINE, ZINC ION, ... (5 entities in total)
• 機能のキーワード: viral protein
• 由来する生物種: Zika virus (strain Mr 766) (ZIKV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 207602.05

構造登録者

Wang, B.,Tan, X.,Song, J. (登録日: 2016-10-12, 公開日: 2017-02-08, 最終更新日: 2024-03-06)

主引用文献

Wang, B.,Tan, X.F.,Thurmond, S.,Zhang, Z.M.,Lin, A.,Hai, R.,Song, J.
The structure of Zika virus NS5 reveals a conserved domain conformation.
Nat Commun, 8:14763-14763, 2017

PubMed Abstract: The recent outbreak of Zika virus (ZIKV) has imposed a serious threat to public health. Here we report the crystal structure of the ZIKV NS5 protein in complex with S-adenosyl-L-homocysteine, in which the tandem methyltransferase (MTase) and RNA-dependent RNA polymerase (RdRp) domains stack into one of the two alternative conformations of flavivirus NS5 proteins. The activity of this NS5 protein is verified through a de novo RdRp assay on a subgenomic ZIKV RNA template. Importantly, our structural analysis leads to the identification of a potential drug-binding site of ZIKV NS5, which might facilitate the development of novel antivirals for ZIKV.

PubMed: 28345600
DOI: 10.1038/ncomms14763

実験手法

X-RAY DIFFRACTION (3.278 Å)