5TK0

Lysozyme structure collected with 3D printed polymer mounts

5TK0 の概要

• エントリーDOI: 10.2210/pdb5tk0/pdb
• 分子名称: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: lysozyme, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14276.44

構造登録者

Kilah, N.L.,MacDonald, N.,Bunton, G.,Park, A.Y.,Breadmore, M.C. (登録日: 2016-10-06, 公開日: 2017-04-05, 最終更新日: 2024-10-23)

主引用文献

Macdonald, N.P.,Bunton, G.L.,Park, A.Y.,Breadmore, M.C.,Kilah, N.L.
3D Printed Micrometer-Scale Polymer Mounts for Single Crystal Analysis.
Anal. Chem., 89:4405-4408, 2017

PubMed Abstract: 3D printed micrometer-scale polymer mounts for single crystal analysis have been prepared by photopolymerization using digital light projection stereolithography (DLP-SLA), with a commercially available digital light projection stereolithography printer (US$4000) and 3DM-ABS resin (US$150 per liter). The polymer mounts were prepared in batches of 49 in 1 h 15 min, which allowed for rapid prototyping and testing of new crystal mounting designs, with a resin cost of 0.2¢ US per mount. The suitability of the 3D printed mounts for single crystal crystallography has been demonstrated through their use in Cu Kα X-ray diffraction experiments of Rochelle salt (sodium potassium tartrate), the protein lysozyme, and has been employed for routine crystallographic analysis of organic and inorganic materials.

PubMed: 28319372
DOI: 10.1021/acs.analchem.7b00443

実験手法

X-RAY DIFFRACTION (1.795 Å)