5TJW

Influenza A virus Nucleoprotein in Complex with Inhibitory Nanobody

5TJW の概要

• エントリーDOI: 10.2210/pdb5tjw/pdb
• 分子名称: Nucleoprotein, NP-specific inhibitory VHH (2 entities in total)
• 機能のキーワード: influenza, nucleoprotein, vhh, inhibitory, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Influenza A virus (A/WSN/1933(H1N1)); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72517.20

構造登録者

Hanke, L.,Knockenhauer, K.E.,Ploegh, H.L.,Schwartz, T.U. (登録日: 2016-10-05, 公開日: 2016-12-21, 最終更新日: 2024-10-16)

主引用文献

Hanke, L.,Knockenhauer, K.E.,Brewer, R.C.,van Diest, E.,Schmidt, F.I.,Schwartz, T.U.,Ploegh, H.L.
The Antiviral Mechanism of an Influenza A Virus Nucleoprotein-Specific Single-Domain Antibody Fragment.
MBio, 7:-, 2016

PubMed Abstract: Alpaca-derived single-domain antibody fragments (VHHs) that target the influenza A virus nucleoprotein (NP) can protect cells from infection when expressed in the cytosol. We found that one such VHH, αNP-VHH1, exhibits antiviral activity similar to that of Mx proteins by blocking nuclear import of incoming viral ribonucleoproteins (vRNPs) and viral transcription and replication in the nucleus. We determined a 3.2-Å crystal structure of αNP-VHH1 in complex with influenza A virus NP. The VHH binds to a nonconserved region on the body domain of NP, which has been associated with binding to host factors and serves as a determinant of host range. Several of the NP/VHH interface residues determine sensitivity of NP to antiviral Mx GTPases. The structure of the NP/αNP-VHH1 complex affords a plausible explanation for the inhibitory properties of the VHH and suggests a rationale for the antiviral properties of Mx proteins. Such knowledge can be leveraged for much-needed novel antiviral strategies.

PubMed: 27965447
DOI: 10.1128/mBio.01569-16

実験手法

X-RAY DIFFRACTION (3.231 Å)