5T96

Crystal structure of the infectious salmon anemia virus (ISAV) HE viral receptor complex

5T96 の概要

• エントリーDOI: 10.2210/pdb5t96/pdb
• 関連するPDBエントリー: 5T9Y
• 分子名称: HE protein, MAGNESIUM ION, 4-O-acetyl-5-acetamido-3,5-dideoxy-L-glycero-alpha-D-galacto-non-2-ulopyranosonic acid, ... (6 entities in total)
• 機能のキーワード: 4-0-acetylsialic acid, hydrolase, hemagglutinin, coiled-coil, viral receptor-complex, infectious salmon anemia virus, isav, viral protein
• 由来する生物種: Infectious salmon anemia virus
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 229450.59

構造登録者

Cook, J.D.,Sultana, A.,Lee, J.E. (登録日: 2016-09-09, 公開日: 2017-03-22, 最終更新日: 2024-11-13)

主引用文献

Cook, J.D.,Sultana, A.,Lee, J.E.
Structure of the infectious salmon anemia virus receptor complex illustrates a unique binding strategy for attachment.
Proc. Natl. Acad. Sci. U.S.A., 114:E2929-E2936, 2017

PubMed Abstract: Orthomyxoviruses are an important family of RNA viruses, which include the various influenza viruses. Despite global efforts to eradicate orthomyxoviral pathogens, these infections remain pervasive. One such orthomyxovirus, infectious salmon anemia virus (ISAV), spreads easily throughout farmed and wild salmonids, constituting a significant economic burden. ISAV entry requires the interplay of the virion-attached hemagglutinin-esterase and fusion glycoproteins. Preventing infections will rely on improved understanding of ISAV entry. Here, we present the crystal structures of ISAV hemagglutinin-esterase unbound and complexed with receptor. Several distinctive features observed in ISAV HE are not seen in any other viral glycoprotein. The structures reveal a unique mode of receptor binding that is dependent on the oligomeric assembly of hemagglutinin-esterase. Importantly, ISAV hemagglutinin-esterase receptor engagement does not initiate conformational rearrangements, suggesting a distinct viral entry mechanism. This work improves our understanding of ISAV pathogenesis and expands our knowledge on the overall diversity of viral glycoprotein-mediated entry mechanisms. Finally, it provides an atomic-resolution model of the primary neutralizing antigen critical for vaccine development.

PubMed: 28320973
DOI: 10.1073/pnas.1617993114

実験手法

X-RAY DIFFRACTION (2 Å)