5T5H

Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit

This is a non-PDB format compatible entry.

Summary for 5T5H

• Entry DOI: 10.2210/pdb5t5h/pdb
• EMDB information: 8361
• Descriptor: RNA LARGE SUBUNIT ALPHA, 60S ribosomal protein L11, 60S ribosomal protein L13, ... (49 entities in total)
• Functional Keywords: trypanosoma cruzi, ribosomal subunit, assmebly, multiply fragmented rrnas, 2.5 angstrom cryo-em structure, ribosome
• Biological source: Trypanosoma cruzi; More
• Total number of polymer chains: 46
• Total formula weight: 1591694.08

Authors

Liu, Z.,Gutierrez-Vargas, C.,Wei, J.,Grassucci, R.A.,Ramesh, M.,Espina, N.,Sun, M.,Tutuncuoglu, B.,Madison-Antenucci, S.,Woolford Jr., J.L.,Tong, L.,Frank, J. (deposition date: 2016-08-31, release date: 2016-10-12, Last modification date: 2019-12-18)

Primary citation

Liu, Z.,Gutierrez-Vargas, C.,Wei, J.,Grassucci, R.A.,Ramesh, M.,Espina, N.,Sun, M.,Tutuncuoglu, B.,Madison-Antenucci, S.,Woolford, J.L.,Tong, L.,Frank, J.
Structure and assembly model for the Trypanosoma cruzi 60S ribosomal subunit.
Proc.Natl.Acad.Sci.USA, 113:12174-12179, 2016

PubMed Abstract: Ribosomes of trypanosomatids, a family of protozoan parasites causing debilitating human diseases, possess multiply fragmented rRNAs that together are analogous to 28S rRNA, unusually large rRNA expansion segments, and r-protein variations compared with other eukaryotic ribosomes. To investigate the architecture of the trypanosomatid ribosomes, we determined the 2.5-Å structure of the Trypanosoma cruzi ribosome large subunit by single-particle cryo-EM. Examination of this structure and comparative analysis of the yeast ribosomal assembly pathway allowed us to develop a stepwise assembly model for the eight pieces of the large subunit rRNAs and a number of ancillary "glue" proteins. This model can be applied to the characterization of Trypanosoma brucei and Leishmania spp. ribosomes as well. Together with other details, our atomic-level structure may provide a foundation for structure-based design of antitrypanosome drugs.

PubMed: 27791004
DOI: 10.1073/pnas.1614594113

Experimental method

ELECTRON MICROSCOPY (2.54 Å)