5T5A

Crystal Structure of the Twister Sister (TS) Ribozyme at 2.0 Angstrom

5T5A の概要

• エントリーDOI: 10.2210/pdb5t5a/pdb
• 分子名称: DNA/RNA (71-MER), MAGNESIUM ION (3 entities in total)
• 機能のキーワード: rna junction catalysis, rna
• 由来する生物種: metagenome; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20355.50

構造登録者

Lilley, D.M.J.,Liu, Y.,Wilson, T.J. (登録日: 2016-08-30, 公開日: 2017-03-08, 最終更新日: 2024-05-08)

主引用文献

Liu, Y.,Wilson, T.J.,Lilley, D.M.
The structure of a nucleolytic ribozyme that employs a catalytic metal ion.
Nat. Chem. Biol., 13:508-513, 2017

PubMed Abstract: The TS ribozyme (originally called "twister sister") is a catalytic RNA. We present a crystal structure of the ribozyme in a pre-reactive conformation. Two co-axial helical stacks are organized by a three-way junction and two tertiary contacts. Five divalent metal ions are directly coordinated to RNA ligands, making important contributions to the RNA architecture. The scissile phosphate lies in a quasihelical loop region that is organized by a network of hydrogen bonding. A divalent metal ion is directly bound to the nucleobase 5' to the scissile phosphate, with an inner-sphere water molecule positioned to interact with the O2' nucleophile. The rate of ribozyme cleavage correlated in a log-linear manner with divalent metal ion pK, consistent with proton transfer in the transition state, and we propose that the bound metal ion is a likely general base for the cleavage reaction. Our data indicate that the TS ribozyme functions predominantly as a metalloenzyme.

PubMed: 28263963
DOI: 10.1038/nchembio.2333

実験手法

X-RAY DIFFRACTION (2 Å)