5SYN

Cocrystal structure of the human acyl protein thioesterase 2 with an isoform-selective inhibitor, ML349

5SYN の概要

• エントリーDOI: 10.2210/pdb5syn/pdb
• 関連するPDBエントリー: 5SYM
• 分子名称: Acyl-protein thioesterase 2, 2-[4-(4-methoxyphenyl)piperazine-1-carbonyl]-5lambda~6~-thieno[3,2-c][1]benzothiopyran-5,5(4H)-dione, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: hydrolase, inhibitor, thioesterase, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101250.49

構造登録者

Stuckey, J.A.,Labby, K.J.,Meagher, J.L.,Won, S.J.,Martin, B.R. (登録日: 2016-08-11, 公開日: 2016-10-26, 最終更新日: 2023-10-04)

主引用文献

Won, S.J.,Davda, D.,Labby, K.J.,Hwang, S.Y.,Pricer, R.,Majmudar, J.D.,Armacost, K.A.,Rodriguez, L.A.,Rodriguez, C.L.,Chong, F.S.,Torossian, K.A.,Palakurthi, J.,Hur, E.S.,Meagher, J.L.,Brooks, C.L.,Stuckey, J.A.,Martin, B.R.
Molecular Mechanism for Isoform-Selective Inhibition of Acyl Protein Thioesterases 1 and 2 (APT1 and APT2).
ACS Chem. Biol., 11:3374-3382, 2016

PubMed Abstract: Post-translational S-palmitoylation directs the trafficking and membrane localization of hundreds of cellular proteins, often involving a coordinated palmitoylation cycle that requires both protein acyl transferases (PATs) and acyl protein thioesterases (APTs) to actively redistribute S-palmitoylated proteins toward different cellular membrane compartments. This process is necessary for the trafficking and oncogenic signaling of S-palmitoylated Ras isoforms, and potentially many peripheral membrane proteins. The depalmitoylating enzymes APT1 and APT2 are separately conserved in all vertebrates, suggesting unique functional roles for each enzyme. The recent discovery of the APT isoform-selective inhibitors ML348 and ML349 has opened new possibilities to probe the function of each enzyme, yet it remains unclear how each inhibitor achieves orthogonal inhibition. Herein, we report the high-resolution structure of human APT2 in complex with ML349 (1.64 Å), as well as the complementary structure of human APT1 bound to ML348 (1.55 Å). Although the overall peptide backbone structures are nearly identical, each inhibitor adopts a distinct conformation within each active site. In APT1, the trifluoromethyl group of ML348 is positioned above the catalytic triad, but in APT2, the sulfonyl group of ML349 forms hydrogen bonds with active site resident waters to indirectly engage the catalytic triad and oxyanion hole. Reciprocal mutagenesis and activity profiling revealed several differing residues surrounding the active site that serve as critical gatekeepers for isoform accessibility and dynamics. Structural and biochemical analysis suggests the inhibitors occupy a putative acyl-binding region, establishing the mechanism for isoform-specific inhibition, hydrolysis of acyl substrates, and structural orthogonality important for future probe development.

PubMed: 27748579
DOI: 10.1021/acschembio.6b00720

実験手法

X-RAY DIFFRACTION (1.64 Å)