5SWI

Crystal structure of SpGH92 in complex with mannose

Summary for 5SWI

• Entry DOI: 10.2210/pdb5swi/pdb
• Related: 5SWA 5SWB
• Descriptor: Sugar hydrolase, CALCIUM ION, GLYCEROL, ... (5 entities in total)
• Functional Keywords: beta sandwich, (alpha/alpha)6 barrel, glycoside hydrolase, hydrolase
• Biological source: Streptococcus pneumoniae
• Total number of polymer chains: 4
• Total formula weight: 328659.60

Authors

Shapiro-Ward, S.,Boraston, A.B. (deposition date: 2016-08-08, release date: 2016-12-14, Last modification date: 2023-10-04)

Primary citation

Robb, M.,Hobbs, J.K.,Woodiga, S.A.,Shapiro-Ward, S.,Suits, M.D.,McGregor, N.,Brumer, H.,Yesilkaya, H.,King, S.J.,Boraston, A.B.
Molecular Characterization of N-glycan Degradation and Transport in Streptococcus pneumoniae and Its Contribution to Virulence.
PLoS Pathog., 13:e1006090-e1006090, 2017

PubMed Abstract: The carbohydrate-rich coating of human tissues and cells provide a first point of contact for colonizing and invading bacteria. Commensurate with N-glycosylation being an abundant form of protein glycosylation that has critical functional roles in the host, some host-adapted bacteria possess the machinery to process N-linked glycans. The human pathogen Streptococcus pneumoniae depolymerizes complex N-glycans with enzymes that sequentially trim a complex N-glycan down to the Man3GlcNAc2 core prior to the release of the glycan from the protein by endo-β-N-acetylglucosaminidase (EndoD), which cleaves between the two GlcNAc residues. Here we examine the capacity of S. pneumoniae to process high-mannose N-glycans and transport the products. Through biochemical and structural analyses we demonstrate that S. pneumoniae also possesses an α-(1,2)-mannosidase (SpGH92). This enzyme has the ability to trim the terminal α-(1,2)-linked mannose residues of high-mannose N-glycans to generate Man5GlcNAc2. Through this activity SpGH92 is able to produce a substrate for EndoD, which is not active on high-mannose glycans with α-(1,2)-linked mannose residues. Binding studies and X-ray crystallography show that NgtS, the solute binding protein of an ABC transporter (ABCNG), is able to bind Man5GlcNAc, a product of EndoD activity, with high affinity. Finally, we evaluated the contribution of EndoD and ABCNG to growth of S. pneumoniae on a model N-glycosylated glycoprotein, and the contribution of these enzymes and SpGH92 to virulence in a mouse model. We found that both EndoD and ABCNG contribute to growth of S. pneumoniae, but that only SpGH92 and EndoD contribute to virulence. Therefore, N-glycan processing, but not transport of the released glycan, is required for full virulence in S. pneumoniae. To conclude, we synthesize our findings into a model of N-glycan processing by S. pneumoniae in which both complex and high-mannose N-glycans are targeted, and in which the two arms of this degradation pathway converge at ABCNG.

PubMed: 28056108
DOI: 10.1371/journal.ppat.1006090

Experimental method

X-RAY DIFFRACTION (2.15 Å)