5SVD

Nop9, a new PUF-like protein, prevents premature pre-rRNA cleavage to correctly process mature 18S rRNA

5SVD の概要

• エントリーDOI: 10.2210/pdb5svd/pdb
• 分子名称: Nucleolar protein 9 (2 entities in total)
• 機能のキーワード: nop9, puf-like proteins, its1 ribosome biogenesis, rna-binding protein, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 139894.98

構造登録者

Zhang, J.,Qiu, C.,Hall, T. (登録日: 2016-08-05, 公開日: 2016-10-26, 最終更新日: 2024-03-06)

主引用文献

Zhang, J.,McCann, K.L.,Qiu, C.,Gonzalez, L.E.,Baserga, S.J.,Hall, T.M.
Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA.
Nat Commun, 7:13085-13085, 2016

PubMed Abstract: Numerous factors direct eukaryotic ribosome biogenesis, and defects in a single ribosome assembly factor may be lethal or produce tissue-specific human ribosomopathies. Pre-ribosomal RNAs (pre-rRNAs) must be processed stepwise and at the correct subcellular locations to produce the mature rRNAs. Nop9 is a conserved small ribosomal subunit biogenesis factor, essential in yeast. Here we report a 2.1-Å crystal structure of Nop9 and a small-angle X-ray-scattering model of a Nop9:RNA complex that reveals a 'C'-shaped fold formed from 11 Pumilio repeats. We show that Nop9 recognizes sequence and structural features of the 20S pre-rRNA near the cleavage site of the nuclease, Nob1. We further demonstrate that Nop9 inhibits Nob1 cleavage, the final processing step to produce mature small ribosomal subunit 18S rRNA. Together, our results suggest that Nop9 is critical for timely cleavage of the 20S pre-rRNA. Moreover, the Nop9 structure exemplifies a new class of Pumilio repeat proteins.

PubMed: 27725644
DOI: 10.1038/ncomms13085

実験手法

X-RAY DIFFRACTION (2.1 Å)