5S5I

Tubulin-Z295848548-complex

5S5I の概要

• エントリーDOI: 10.2210/pdb5s5i/pdb
• Group deposition: XChem fragment screening on T2R-TTL (G_1002173)
• 分子名称: Tubulin alpha-1B chain, 4-[(3-cyclopropyl-1,2,4-oxadiazol-5-yl)methyl]morpholine, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, ... (12 entities in total)
• 機能のキーワード: cell cycle, tubulin fold, cytoskeleton, microtubule
• 由来する生物種: Rattus norvegicus (Rat); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 264755.61

構造登録者

Muehlethaler, T.,Gioia, D.,Prota, A.E.,Sharpe, M.E.,Cavalli, A.,Steinmetz, M.O. (登録日: 2020-11-08, 公開日: 2021-06-30, 最終更新日: 2024-03-06)

主引用文献

Muhlethaler, T.,Gioia, D.,Prota, A.E.,Sharpe, M.E.,Cavalli, A.,Steinmetz, M.O.
Comprehensive Analysis of Binding Sites in Tubulin.
Angew.Chem.Int.Ed.Engl., 60:13331-13342, 2021

PubMed Abstract: Tubulin plays essential roles in vital cellular activities and is the target of a wide range of proteins and ligands. Here, using a combined computational and crystallographic fragment screening approach, we addressed the question of how many binding sites exist in tubulin. We identified 27 distinct sites, of which 11 have not been described previously, and analyzed their relationship to known tubulin-protein and tubulin-ligand interactions. We further observed an intricate pocket communication network and identified 56 chemically diverse fragments that bound to 10 distinct tubulin sites. Our results offer a unique structural basis for the development of novel small molecules for use as tubulin modulators in basic research applications or as drugs. Furthermore, our method lays down a framework that may help to discover new pockets in other pharmaceutically important targets and characterize them in terms of chemical tractability and allosteric modulation.

PubMed: 33951246
DOI: 10.1002/anie.202100273

実験手法

X-RAY DIFFRACTION (2.49 Å)