5OSH

Structure of retromer VPS29-VPS35C subunits complexed with RidL N-terminal domain (1-236)

5OSH の概要

• エントリーDOI: 10.2210/pdb5osh/pdb
• 分子名称: Vacuolar protein sorting-associated protein 29, Vacuolar protein sorting-associated protein 35, Interaptin (3 entities in total)
• 機能のキーワード: retromer, legionella pneumophila, transport protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 322499.23

構造登録者

Romano-Moreno, M.,Rojas, A.L.,Lucas, M.,Isupov, M.N.,Hierro, A. (登録日: 2017-08-17, 公開日: 2017-12-13, 最終更新日: 2024-05-08)

主引用文献

Romano-Moreno, M.,Rojas, A.L.,Williamson, C.D.,Gershlick, D.C.,Lucas, M.,Isupov, M.N.,Bonifacino, J.S.,Machner, M.P.,Hierro, A.
Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL.
Proc. Natl. Acad. Sci. U.S.A., 114:E11151-E11160, 2017

PubMed Abstract: Microbial pathogens employ sophisticated virulence strategies to cause infections in humans. The intracellular pathogen encodes RidL to hijack the host scaffold protein VPS29, a component of retromer and retriever complexes critical for endosomal cargo recycling. Here, we determined the crystal structure of RidL in complex with the human VPS29-VPS35 retromer subcomplex. A hairpin loop protruding from RidL inserts into a conserved pocket on VPS29 that is also used by cellular ligands, such as Tre-2/Bub2/Cdc16 domain family member 5 (TBC1D5) and VPS9-ankyrin repeat protein for VPS29 binding. Consistent with the idea of molecular mimicry in protein interactions, RidL outcompeted TBC1D5 for binding to VPS29. Furthermore, the interaction of RidL with retromer did not interfere with retromer dimerization but was essential for association of RidL with retromer-coated vacuolar and tubular endosomes. Our work thus provides structural and mechanistic evidence into how RidL is targeted to endosomal membranes.

PubMed: 29229824
DOI: 10.1073/pnas.1715361115

実験手法

X-RAY DIFFRACTION (4.3 Å)