5OR3

Crystal structure of Aspergillus oryzae catechol oxidase in met/deoxy-form

5OR3 の概要

• エントリーDOI: 10.2210/pdb5or3/pdb
• 関連するPDBエントリー: 5or3
• 分子名称: catechol oxidase, PEROXIDE ION, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: catechol oxidase, polyphenol oxidase, coupled binuclear copper enzyme, type 3 copper center, oxidoreductase
• 由来する生物種: Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 174911.49

構造登録者

Hakulinen, N.,Penttinen, L.,Rutanen, C.,Rouvinen, J. (登録日: 2017-08-15, 公開日: 2018-05-09, 最終更新日: 2024-11-20)

主引用文献

Penttinen, L.,Rutanen, C.,Saloheimo, M.,Kruus, K.,Rouvinen, J.,Hakulinen, N.
A new crystal form of Aspergillus oryzae catechol oxidase and evaluation of copper site structures in coupled binuclear copper enzymes.
PLoS ONE, 13:e0196691-e0196691, 2018

PubMed Abstract: Coupled binuclear copper (CBC) enzymes have a conserved type 3 copper site that binds molecular oxygen to oxidize various mono- and diphenolic compounds. In this study, we found a new crystal form of catechol oxidase from Aspergillus oryzae (AoCO4) and solved two new structures from two different crystals at 1.8-Å and at 2.5-Å resolutions. These structures showed different copper site forms (met/deoxy and deoxy) and also differed from the copper site observed in the previously solved structure of AoCO4. We also analysed the electron density maps of all of the 56 CBC enzyme structures available in the protein data bank (PDB) and found that many of the published structures have vague copper sites. Some of the copper sites were then re-refined to find a better fit to the observed electron density. General problems in the refinement of metalloproteins and metal centres are discussed.

PubMed: 29715329
DOI: 10.1371/journal.pone.0196691

実験手法

X-RAY DIFFRACTION (1.795 Å)