5OLP

Galacturonidase

5OLP の概要

• エントリーDOI: 10.2210/pdb5olp/pdb
• 分子名称: Pectate lyase, CALCIUM ION (3 entities in total)
• 機能のキーワード: galacturonidase involved in the degradation of dietary pectic glycans in the human gut., hydrolase
• 由来する生物種: Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 103308.50

構造登録者

Basle, A.,Luis, A.S.,Gilbert, H.J. (登録日: 2017-07-28, 公開日: 2017-11-29, 最終更新日: 2024-11-20)

主引用文献

Luis, A.S.,Briggs, J.,Zhang, X.,Farnell, B.,Ndeh, D.,Labourel, A.,Basle, A.,Cartmell, A.,Terrapon, N.,Stott, K.,Lowe, E.C.,McLean, R.,Shearer, K.,Schuckel, J.,Venditto, I.,Ralet, M.C.,Henrissat, B.,Martens, E.C.,Mosimann, S.C.,Abbott, D.W.,Gilbert, H.J.
Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides.
Nat Microbiol, 3:210-219, 2018

PubMed Abstract: The major nutrients available to human colonic Bacteroides species are glycans, exemplified by pectins, a network of covalently linked plant cell wall polysaccharides containing galacturonic acid (GalA). Metabolism of complex carbohydrates by the Bacteroides genus is orchestrated by polysaccharide utilization loci (PULs). In Bacteroides thetaiotaomicron, a human colonic bacterium, the PULs activated by different pectin domains have been identified; however, the mechanism by which these loci contribute to the degradation of these GalA-containing polysaccharides is poorly understood. Here we show that each PUL orchestrates the metabolism of specific pectin molecules, recruiting enzymes from two previously unknown glycoside hydrolase families. The apparatus that depolymerizes the backbone of rhamnogalacturonan-I is particularly complex. This system contains several glycoside hydrolases that trim the remnants of other pectin domains attached to rhamnogalacturonan-I, and nine enzymes that contribute to the degradation of the backbone that makes up a rhamnose-GalA repeating unit. The catalytic properties of the pectin-degrading enzymes are optimized to protect the glycan cues that activate the specific PULs ensuring a continuous supply of inducing molecules throughout growth. The contribution of Bacteroides spp. to metabolism of the pectic network is illustrated by cross-feeding between organisms.

PubMed: 29255254
DOI: 10.1038/s41564-017-0079-1

実験手法

X-RAY DIFFRACTION (2 Å)