5OLC

Crystal structure of the 3,6-anhydro-D-galactonate cycloisomerase from Zobellia galactanivorans

Summary for 5OLC

• Entry DOI: 10.2210/pdb5olc/pdb
• Descriptor: Galactonate dehydratase, MAGNESIUM ION (3 entities in total)
• Functional Keywords: cycloisomerase, enolase superfamily, 3, 6-anhydro-d-galactonate, carrageenan, isomerase
• Biological source: Zobellia galactanivorans
• Total number of polymer chains: 8
• Total formula weight: 359301.03

Authors

Michel, G.,Czjzek, M.,Jam, M. (deposition date: 2017-07-27, release date: 2017-12-06, Last modification date: 2024-01-17)

Primary citation

Ficko-Blean, E.,Prechoux, A.,Thomas, F.,Rochat, T.,Larocque, R.,Zhu, Y.,Stam, M.,Genicot, S.,Jam, M.,Calteau, A.,Viart, B.,Ropartz, D.,Perez-Pascual, D.,Correc, G.,Matard-Mann, M.,Stubbs, K.A.,Rogniaux, H.,Jeudy, A.,Barbeyron, T.,Medigue, C.,Czjzek, M.,Vallenet, D.,McBride, M.J.,Duchaud, E.,Michel, G.
Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria.
Nat Commun, 8:1685-1685, 2017

PubMed Abstract: Macroalgae contribute substantially to primary production in coastal ecosystems. Their biomass, mainly consisting of polysaccharides, is cycled into the environment by marine heterotrophic bacteria using largely uncharacterized mechanisms. Here we describe the complete catabolic pathway for carrageenans, major cell wall polysaccharides of red macroalgae, in the marine heterotrophic bacterium Zobellia galactanivorans. Carrageenan catabolism relies on a multifaceted carrageenan-induced regulon, including a non-canonical polysaccharide utilization locus (PUL) and genes distal to the PUL, including a susCD-like pair. The carrageenan utilization system is well conserved in marine Bacteroidetes but modified in other phyla of marine heterotrophic bacteria. The core system is completed by additional functions that might be assumed by non-orthologous genes in different species. This complex genetic structure may be the result of multiple evolutionary events including gene duplications and horizontal gene transfers. These results allow for an extension on the definition of bacterial PUL-mediated polysaccharide digestion.

PubMed: 29162826
DOI: 10.1038/s41467-017-01832-6

Experimental method

X-RAY DIFFRACTION (2.79 Å)