5OHO

Crystal structure of the KOWx-KOW4 domain of human DSIF

5OHO の概要

• エントリーDOI: 10.2210/pdb5oho/pdb
• 関連するPDBエントリー: 5OHQ 5OIK
• EMDBエントリー: 3815 3816 3817 3818 3819
• 分子名称: Transcription elongation factor SPT5, GLYCEROL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: rna polymerase ii, transcription elongation, transcription
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26197.81

構造登録者

Bernecky, C.,Plitzko, J.M.,Cramer, P. (登録日: 2017-07-17, 公開日: 2017-09-13, 最終更新日: 2024-05-08)

主引用文献

Bernecky, C.,Plitzko, J.M.,Cramer, P.
Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp.
Nat. Struct. Mol. Biol., 24:809-815, 2017

PubMed Abstract: During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation complex at a nominal resolution of 3.4 Å. Human DSIF has a modular structure with two domains forming a DNA clamp, two domains forming an RNA clamp, and one domain buttressing the RNA clamp. The clamps maintain the transcription bubble, position upstream DNA, and retain the RNA transcript in the exit tunnel. The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. The structure provides insight into the roles of DSIF during mRNA synthesis.

PubMed: 28892040
DOI: 10.1038/nsmb.3465

実験手法

X-RAY DIFFRACTION (1.601 Å)