5ODK

Single-stranded DNA-binding protein from bacteriophage Enc34, C-terminal truncation

5ODK の概要

• エントリーDOI: 10.2210/pdb5odk/pdb
• 関連するPDBエントリー: 5ODJ 5ODK
• 分子名称: single-stranded DNA-binding protein, PHOSPHATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: dna replication, duf2815, ssb, ob fold, dna binding protein
• 由来する生物種: Enterobacter phage Enc34
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60963.60

構造登録者

Cernooka, E.,Rumnieks, J.,Kazaks, A.,Tars, K. (登録日: 2017-07-05, 公開日: 2017-10-25, 最終更新日: 2024-01-17)

主引用文献

Cernooka, E.,Rumnieks, J.,Tars, K.,Kazaks, A.
Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34.
Sci Rep, 7:15529-15529, 2017

PubMed Abstract: Modern DNA sequencing capabilities have led to the discovery of a large number of new bacteriophage genomes, which are a rich source of novel proteins with an unidentified biological role. The genome of Enterobacter cancerogenus bacteriophage Enc34 contains several proteins of unknown function that are nevertheless conserved among distantly related phages. Here, we report the crystal structure of a conserved Enc34 replication protein ORF6 which contains a domain of unknown function DUF2815. Despite the low (~15%) sequence identity, the Enc34 ORF6 structurally resembles the gene 2.5 protein from bacteriophage T7, and likewise is a single-stranded DNA (ssDNA)-binding protein (SSB) that consists of a variation of the oligosaccharide/oligonucleotide-binding (OB)-fold and an unstructured C-terminal segment. We further report the crystal structure of a C-terminally truncated ORF6 in complex with an ssDNA oligonucleotide that reveals a DNA-binding mode involving two aromatic stacks and multiple electrostatic interactions, with implications for a common ssDNA recognition mechanism for all T7-type SSBs.

PubMed: 29138440
DOI: 10.1038/s41598-017-15774-y

実験手法

X-RAY DIFFRACTION (1.34 Å)