5ODG

Crystal structure of Smad3-MH1 bound to the GGCT site.

5ODG の概要

• エントリーDOI: 10.2210/pdb5odg/pdb
• 関連するPDBエントリー: 5OD6
• 分子名称: Mothers against decapentaplegic homolog 3, DNA (5'-D(P*CP*AP*GP*GP*CP*TP*AP*GP*CP*CP*TP*GP*CP*A)-3'), ZINC ION, ... (5 entities in total)
• 機能のキーワード: smads, transcription factor, dna complex, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm : P84022
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 40037.85

構造登録者

Kaczmarska, Z.,Marquez, J.A.,Macias, M.J. (登録日: 2017-07-05, 公開日: 2017-11-15, 最終更新日: 2024-05-08)

主引用文献

Martin-Malpartida, P.,Batet, M.,Kaczmarska, Z.,Freier, R.,Gomes, T.,Aragon, E.,Zou, Y.,Wang, Q.,Xi, Q.,Ruiz, L.,Vea, A.,Marquez, J.A.,Massague, J.,Macias, M.J.
Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors.
Nat Commun, 8:2070-2070, 2017

PubMed Abstract: Smad transcription factors activated by TGF-β or by BMP receptors form trimeric complexes with Smad4 to target specific genes for cell fate regulation. The CAGAC motif has been considered as the main binding element for Smad2/3/4, whereas Smad1/5/8 have been thought to preferentially bind GC-rich elements. However, chromatin immunoprecipitation analysis in embryonic stem cells showed extensive binding of Smad2/3/4 to GC-rich cis-regulatory elements. Here, we present the structural basis for specific binding of Smad3 and Smad4 to GC-rich motifs in the goosecoid promoter, a nodal-regulated differentiation gene. The structures revealed a 5-bp consensus sequence GGC(GC)|(CG) as the binding site for both TGF-β and BMP-activated Smads and for Smad4. These 5GC motifs are highly represented as clusters in Smad-bound regions genome-wide. Our results provide a basis for understanding the functional adaptability of Smads in different cellular contexts, and their dependence on lineage-determining transcription factors to target specific genes in TGF-β and BMP pathways.

PubMed: 29234012
DOI: 10.1038/s41467-017-02054-6

実験手法

X-RAY DIFFRACTION (2.12 Å)