5O8N

Structure of thermolysin at room temperature via a method of acoustically induced rotation.

5O8N の概要

• エントリーDOI: 10.2210/pdb5o8n/pdb
• 分子名称: Thermolysin, CALCIUM ION, ZINC ION, ... (6 entities in total)
• 機能のキーワード: hydrolase, zinc protease
• 由来する生物種: Bacillus thermoproteolyticus
• 細胞内の位置: Secreted: P00800
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34807.37

構造登録者

Axford, D.N.,Burton, C.,Docker, P.,Prince, M.,Topham, P.D. (登録日: 2017-06-13, 公開日: 2017-08-09, 最終更新日: 2024-01-17)

主引用文献

Burton, C.G.,Axford, D.,Edwards, A.M.J.,Gildea, R.J.,Morris, R.H.,Newton, M.I.,Orville, A.M.,Prince, M.,Topham, P.D.,Docker, P.T.
An acoustic on-chip goniometer for room temperature macromolecular crystallography.
Lab Chip, 17:4225-4230, 2017

PubMed Abstract: This paper describes the design, development and successful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for gradual in-fluid sample reorientation about varying axes and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facilitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

PubMed: 29124258
DOI: 10.1039/c7lc00812k

実験手法

X-RAY DIFFRACTION (1.9 Å)