Summary for 5O7X
| Entry DOI | 10.2210/pdb5o7x/pdb |
| Descriptor | RNA polymerase I-specific transcription initiation factor RRN6, RNA polymerase I-specific transcription initiation factor RRN7, RNA polymerase I-specific transcription initiation factor RRN11, ... (5 entities in total) |
| Functional Keywords | transcription, rna polymerase i initiation, core factor |
| Biological source | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) More |
| Total number of polymer chains | 18 |
| Total formula weight | 1334623.82 |
| Authors | Engel, C.,Gubbey, T.,Neyer, S.,Sainsbury, S.,Oberthuer, C.,Baejen, C.,Bernecky, C.,Cramer, P. (deposition date: 2017-06-09, release date: 2017-08-02, Last modification date: 2024-05-08) |
| Primary citation | Engel, C.,Gubbey, T.,Neyer, S.,Sainsbury, S.,Oberthuer, C.,Baejen, C.,Bernecky, C.,Cramer, P. Structural Basis of RNA Polymerase I Transcription Initiation. Cell, 169:120-131.e22, 2017 Cited by PubMed Abstract: Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase. PubMed: 28340337DOI: 10.1016/j.cell.2017.03.003 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.2 Å) |
Structure validation
Download full validation report
