5O7X

CRYSTAL STRUCTURE OF S. CEREVISIAE CORE FACTOR AT 3.2A RESOLUTION

「5N5X」から置き換えられました

5O7X の概要

• エントリーDOI: 10.2210/pdb5o7x/pdb
• 分子名称: RNA polymerase I-specific transcription initiation factor RRN6, RNA polymerase I-specific transcription initiation factor RRN7, RNA polymerase I-specific transcription initiation factor RRN11, ... (5 entities in total)
• 機能のキーワード: transcription, rna polymerase i initiation, core factor
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 1334623.82

構造登録者

Engel, C.,Gubbey, T.,Neyer, S.,Sainsbury, S.,Oberthuer, C.,Baejen, C.,Bernecky, C.,Cramer, P. (登録日: 2017-06-09, 公開日: 2017-08-02, 最終更新日: 2024-05-08)

主引用文献

Engel, C.,Gubbey, T.,Neyer, S.,Sainsbury, S.,Oberthuer, C.,Baejen, C.,Bernecky, C.,Cramer, P.
Structural Basis of RNA Polymerase I Transcription Initiation.
Cell, 169:120-131.e22, 2017

PubMed Abstract: Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.

PubMed: 28340337
DOI: 10.1016/j.cell.2017.03.003

実験手法

X-RAY DIFFRACTION (3.2 Å)