5O7M

Single-shot pink beam serial crystallography: Phycocyanin (One chip, chip_1)

5O7M の概要

• エントリーDOI: 10.2210/pdb5o7m/pdb
• 関連するPDBエントリー: 1MJQ
• 分子名称: C-phycocyanin alpha chain, C-phycocyanin beta chain, PHYCOCYANOBILIN, ... (4 entities in total)
• 機能のキーワード: phycocyanin, serial crystallography, pink beam, photosynthesis
• 由来する生物種: Thermosynechococcus elongatus (strain BP-1); 詳細
• 細胞内の位置: Cellular thylakoid membrane ; Peripheral membrane protein ; Cytoplasmic side : P50032 P50033
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37439.36

構造登録者

Meents, A.,Oberthuer, D.,Lieske, J.,Srajer, V.,Sarrou, I. (登録日: 2017-06-09, 公開日: 2017-11-15, 最終更新日: 2024-01-17)

主引用文献

Meents, A.,Wiedorn, M.O.,Srajer, V.,Henning, R.,Sarrou, I.,Bergtholdt, J.,Barthelmess, M.,Reinke, P.Y.A.,Dierksmeyer, D.,Tolstikova, A.,Schaible, S.,Messerschmidt, M.,Ogata, C.M.,Kissick, D.J.,Taft, M.H.,Manstein, D.J.,Lieske, J.,Oberthuer, D.,Fischetti, R.F.,Chapman, H.N.
Pink-beam serial crystallography.
Nat Commun, 8:1281-1281, 2017

PubMed Abstract: Serial X-ray crystallography allows macromolecular structure determination at both X-ray free electron lasers (XFELs) and, more recently, synchrotron sources. The time resolution for serial synchrotron crystallography experiments has been limited to millisecond timescales with monochromatic beams. The polychromatic, "pink", beam provides a more than two orders of magnitude increased photon flux and hence allows accessing much shorter timescales in diffraction experiments at synchrotron sources. Here we report the structure determination of two different protein samples by merging pink-beam diffraction patterns from many crystals, each collected with a single 100 ps X-ray pulse exposure per crystal using a setup optimized for very low scattering background. In contrast to experiments with monochromatic radiation, data from only 50 crystals were required to obtain complete datasets. The high quality of the diffraction data highlights the potential of this method for studying irreversible reactions at sub-microsecond timescales using high-brightness X-ray facilities.

PubMed: 29097720
DOI: 10.1038/s41467-017-01417-3

実験手法

X-RAY DIFFRACTION (2.46 Å)