5O6V

The cryo-EM structure of Tick-borne encephalitis virus complexed with Fab fragment of neutralizing antibody 19/1786

5O6V の概要

• エントリーDOI: 10.2210/pdb5o6v/pdb
• 関連するPDBエントリー: 5O6A
• EMDBエントリー: 3752 3754
• 分子名称: Envelope protein, Small envelope protein M, Fab 19/1786 - Heavy chain, ... (5 entities in total)
• 機能のキーワード: tick-borne encephalitis virus, fab 19/1786, cryo-em, virus
• 由来する生物種: Tick-borne encephalitis virus (strain Hypr); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 277840.55

構造登録者

Fuzik, T.,Plevka, P. (登録日: 2017-06-07, 公開日: 2018-02-07, 最終更新日: 2024-10-16)

主引用文献

Fuzik, T.,Formanova, P.,Ruzek, D.,Yoshii, K.,Niedrig, M.,Plevka, P.
Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody.
Nat Commun, 9:436-436, 2018

PubMed Abstract: Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. Here, we present cryo-EM structures of the native TBEV virion and its complex with Fab fragments of neutralizing antibody 19/1786. Flavivirus genome delivery depends on membrane fusion that is triggered at low pH. The virion structure indicates that the repulsive interactions of histidine side chains, which become protonated at low pH, may contribute to the disruption of heterotetramers of the TBEV envelope and membrane proteins and induce detachment of the envelope protein ectodomains from the virus membrane. The Fab fragments bind to 120 out of the 180 envelope glycoproteins of the TBEV virion. Unlike most of the previously studied flavivirus-neutralizing antibodies, the Fab fragments do not lock the E-proteins in the native-like arrangement, but interfere with the process of virus-induced membrane fusion.

PubMed: 29382836
DOI: 10.1038/s41467-018-02882-0

実験手法

ELECTRON MICROSCOPY (3.9 Å)