5O60

Structure of the 50S large ribosomal subunit from Mycobacterium smegmatis

Summary for 5O60

• Entry DOI: 10.2210/pdb5o60/pdb
• Related: 5O5J 5O61
• EMDB information: 3748 3750 3751
• Descriptor: 50S ribosomal protein bL37, 50S ribosomal protein L10, 50S ribosomal protein L11, ... (38 entities in total)
• Functional Keywords: ribosome, translation
• Biological source: Mycobacterium smegmatis str. MC2 155; More
• Total number of polymer chains: 35
• Total formula weight: 1496821.68

Authors

Hentschel, J.,Burnside, C.,Mignot, I.,Leibundgut, M.,Boehringer, D.,Ban, N. (deposition date: 2017-06-02, release date: 2017-07-12, Last modification date: 2024-05-15)

Primary citation

Hentschel, J.,Burnside, C.,Mignot, I.,Leibundgut, M.,Boehringer, D.,Ban, N.
The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.
Cell Rep, 20:149-160, 2017

PubMed Abstract: The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-Å cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics.

PubMed: 28683309
DOI: 10.1016/j.celrep.2017.06.029

Experimental method

ELECTRON MICROSCOPY (3.18 Å)